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| Boilerpipe Text | A meta-analysis published this week in BMJ shows that COVID-19 rapid antigen tests for children don't meet minimum performance standards set by the World Health Organization (WHO) and the US Food and Drug Administration (FDA). Already shown to be far less sensitive in adults than their manufacturers report, rapid antigen tests, also called lateral flow tests, are widely used in children to screen for COVID-19 in schools, homes, and healthcare settings. Amid scarcity of the over-the-counter tests, US households can now order four such tests from a US government website for free. While experts say these nasal-swab testsāsold under brand names such as BinaxNOW and BD Veritorāhave some value in disrupting virus transmission, it's important to understand their limitationsāespecially amid the current surge of cases caused by the highly transmissible and evasive Omicron (B.1.1.529) variant. "I think an important point to remember is that with rapid antigen tests, positive results are generally meaningful, but negative results have to be taken with a grain of salt," Kelly Wroblewski, MPH, director of infectious diseases at the Association of Public Health Laboratories, told CIDRAP News. Michael Osterholm, PhD, MPH, director of the Center for Infectious Disease Research and Policy (CIDRAP) at the University of Minnesota, publisher of CIDRAP News, calls interpreting negative test results "a huge challenge." "The average consumer isn't going to know what this means. Many people will get a negative result and go to dinner with Grandma and Grandpa." Poor sensitivity, acceptable specificity In the first meta-analysis of its kind, a team led by University of Manchester researchers in the United Kingdom evaluated 17 studies involving 6,355 children that compared rapid antigen test results with those from the gold-standard: reverse transcription polymerase chain reaction (RT-PCR) test. The studies assessed eight tests from six different brands. Overall pooled diagnostic sensitivity and specificity of the rapid tests were 64.2% and 99.1%, respectively. In children with symptoms, diagnostic sensitivity and specificity were 71.8% and 98.7%, respectively, and in those without symptoms, sensitivity and specificity were 56.2% and 98.6%, respectively. For reference, WHO and FDA performance standards for rapid antigen tests specify a minimum sensitivity of 80% and a minimum specificityāof 97%. Sensitivity is the probability that a test correctly identifies all positive cases; the higher the sensitivity, the lower the likelihood of false-negative results. Specificity, on the other hand, is the ability to identify those who don't have a condition; the higher the specificity, the lower the risk of false-positive results. Of the 12 peer-reviewed studies in journals and 5 published on preprint servers, only 1 was at low risk of bias. The researchers concluded that the real-world performance of rapid antigen tests in children varied widely, only a few tests had relevant data, and the risk of bias was unclear due to poor reporting. "Evidence gaps identified in this systematic review demonstrate current research needs to support evidence-based decision making," they wrote. "In particular, evidence is needed on the real-life performance of tests in schools (self-testing performed by children) and kindergarten (sample collection in toddlers by laypersons). ⦠Moreover, the implementation of routine audits of testing programmes may allow monitoring of test performance in practice outside of studies." Many questions remain Rapid antigen tests for COVID-19 first became available in May 2020, when the FDA issued emergency use authorization for Quidel Corporation's Sofia 2 SARS Antigen FIA. Since then, dozens of such tests have come to market. They are convenient and can generate results in as fast as 15 minutes. By comparisonāand depending on demand and availabilityāseveral days may pass before more accurate PCR results are available. On Dec 28, 2021, the FDA reported on its website that preliminary research on the performance of rapid antigen tests in patients infected with Omicron suggests that "antigen tests do detect the omicron variant but may have reduced sensitivity," referring to the potential for false-negative results. The FDA should have released clearer information sooner, Osterholm said, citing cases whereby "an individual is negative for multiple days in a lateral flow test, including on the day a PCR test shows that they are, in fact, positive. We're still trying to clarify what the results tells us about one's infection status and whether it tells us anything about how infectious you are and able to transmit the virus." Tests don't eliminate risks The uncertainty about whether negative rapid antigen tests can be trustedāas well as the similarity of COVID-19 symptoms and those caused by allergies or other common infectious diseasesāhas caused confusion for parents trying to make decisions about school and other activities. Wroblewski recommends that parents of symptomatic children who receive a negative result from a rapid antigen test seek a PCR test for confirmation. If a child tests negative on a rapid test and has no symptoms but was exposed to COVID-19 at school or elsewhere, following up with another rapid test a day or two later could be prudent, she said. "I think parents can, to a certain extent, use their judgment," with negative test results, Wroblewski said. "If your kid has the sniffles but no severe symptoms, no fever, you can probably send them to school with a mask. But if they've had an exposure and have a sniffle, maybe just keep them home. Even if it's not COVID, they could have something else that's infectious." However, if children test positive and have symptoms, they should be considered infected and kept home from school and other activities, Osterholm said. The important thing to understand is that rapid tests are not perfect, Wroblewski said. "If your child is asymptomatic and you're using rapid antigen tests to identify those asymptomatic infections before they go to school, see grandparents, or get on a plane, you're not going to eliminate the risk that someone is actually infected and might move to positive in the next 24 hours," she said. "You're mitigating the risk." |
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# High false-negative rate limits value of rapid COVID tests for kids
Mary Van Beusekom \| News Writer \| CIDRAP News
January 21, 2022
[COVID-19](https://www.cidrap.umn.edu/covid-19)


> tatyana\_tomsickova / iStock
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A [meta-analysis](https://ebm.bmj.com/content/early/2022/01/04/bmjebm-2021-111828 "(opens in a new window)") published this week in *BMJ* shows that COVID-19 rapid antigen tests for children don't meet minimum performance standards set by the World Health Organization (WHO) and the US Food and Drug Administration (FDA).
Already shown to be far less sensitive in adults than their manufacturers report, rapid antigen tests, also called lateral flow tests, are widely used in children to screen for COVID-19 in schools, homes, and healthcare settings. Amid scarcity of the over-the-counter tests, US households can now order four such tests from a US government [website](https://www.covidtests.gov/ "(opens in a new window)") for free.
While experts say these nasal-swab testsāsold under brand names such as BinaxNOW and BD Veritorāhave some value in disrupting virus transmission, it's important to understand their limitationsāespecially amid the current surge of cases caused by the highly transmissible and evasive Omicron (B.1.1.529) variant.
"I think an important point to remember is that with rapid antigen tests, positive results are generally meaningful, but negative results have to be taken with a grain of salt," Kelly Wroblewski, MPH, director of infectious diseases at the Association of Public Health Laboratories, told CIDRAP News.
Michael Osterholm, PhD, MPH, director of the Center for Infectious Disease Research and Policy (CIDRAP) at the University of Minnesota, publisher of CIDRAP News, calls interpreting negative test results "a huge challenge."
"The average consumer isn't going to know what this means. Many people will get a negative result and go to dinner with Grandma and Grandpa."
### Poor sensitivity, acceptable specificity
In the first meta-analysis of its kind, a team led by University of Manchester researchers in the United Kingdom evaluated 17 studies involving 6,355 children that compared rapid antigen test results with those from the gold-standard: reverse transcription polymerase chain reaction (RT-PCR) test. The studies assessed eight tests from six different brands.
Overall pooled diagnostic sensitivity and specificity of the rapid tests were 64.2% and 99.1%, respectively. In children with symptoms, diagnostic sensitivity and specificity were 71.8% and 98.7%, respectively, and in those without symptoms, sensitivity and specificity were 56.2% and 98.6%, respectively. For reference, WHO and FDA performance standards for rapid antigen tests specify a minimum sensitivity of 80% and a minimum specificity of 97%.
Sensitivity is the probability that a test correctly identifies all positive cases; the higher the sensitivity, the lower the likelihood of false-negative results. Specificity, on the other hand, is the ability to identify those who don't have a condition; the higher the specificity, the lower the risk of false-positive results.
Of the 12 peer-reviewed studies in journals and 5 published on preprint servers, only 1 was at low risk of bias.
The researchers concluded that the real-world performance of rapid antigen tests in children varied widely, only a few tests had relevant data, and the risk of bias was unclear due to poor reporting.
"Evidence gaps identified in this systematic review demonstrate current research needs to support evidence-based decision making," they wrote. "In particular, evidence is needed on the real-life performance of tests in schools (self-testing performed by children) and kindergarten (sample collection in toddlers by laypersons). ⦠Moreover, the implementation of routine audits of testing programmes may allow monitoring of test performance in practice outside of studies."
### Many questions remain
Rapid antigen tests for COVID-19 first became available in May 2020, when the FDA issued emergency use authorization for Quidel Corporation's Sofia 2 SARS Antigen FIA. Since then, dozens of such tests have come to market.
They are convenient and can generate results in as fast as 15 minutes. By comparisonāand depending on demand and availabilityāseveral days may pass before more accurate PCR results are available.
On Dec 28, 2021, the FDA reported on its [website](https://www.fda.gov/medical-devices/coronavirus-covid-19-and-medical-devices/sars-cov-2-viral-mutations-impact-covid-19-tests#omicronvariantimpact "(opens in a new window)") that preliminary research on the performance of rapid antigen tests in patients infected with Omicron suggests that "antigen tests do detect the omicron variant but may have reduced sensitivity," referring to the potential for false-negative results.
The FDA should have released clearer information sooner, Osterholm said, citing cases whereby "an individual is negative for multiple days in a lateral flow test, including on the day a PCR test shows that they are, in fact, positive. We're still trying to clarify what the results tells us about one's infection status and whether it tells us anything about how infectious you are and able to transmit the virus."
### Tests don't eliminate risks
The uncertainty about whether negative rapid antigen tests can be trustedāas well as the similarity of COVID-19 symptoms and those caused by allergies or other common infectious diseasesāhas caused confusion for parents trying to make decisions about school and other activities.
Wroblewski recommends that parents of symptomatic children who receive a negative result from a rapid antigen test seek a PCR test for confirmation. If a child tests negative on a rapid test and has no symptoms but was exposed to COVID-19 at school or elsewhere, following up with another rapid test a day or two later could be prudent, she said.
"I think parents can, to a certain extent, use their judgment," with negative test results, Wroblewski said. "If your kid has the sniffles but no severe symptoms, no fever, you can probably send them to school with a mask. But if they've had an exposure and have a sniffle, maybe just keep them home. Even if it's not COVID, they could have something else that's infectious."
However, if children test positive and have symptoms, they should be considered infected and kept home from school and other activities, Osterholm said.
The important thing to understand is that rapid tests are not perfect, Wroblewski said. "If your child is asymptomatic and you're using rapid antigen tests to identify those asymptomatic infections before they go to school, see grandparents, or get on a plane, you're not going to eliminate the risk that someone is actually infected and might move to positive in the next 24 hours," she said. "You're mitigating the risk."
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