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URLhttps://authors.library.caltech.edu/records/0mjqz-64458
Last Crawled2026-02-07 20:39:03 (2 months ago)
First Indexed2023-11-18 02:50:08 (2 years ago)
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Meta TitleLaboratory Evaluation Links Some False-Positive COVID-19 Antigen Test Results Observed in a Field Study to a Specific Lot of Test Strips
Meta DescriptionDuring a household-transmission field study using COVID-19 antigen rapid diagnostic tests (Ag-RDT), a common test strip lot was identified among three participants with false-positive results. In blinded laboratory evaluation, this lot, exhibited a significantly higher false-positive rate than other lots. Because a positive Ag-RDT result often prompts action, reducing lot-specific false positives can maintain confidence and actionability of true-positive Ag-RDT results.
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Published January 30, 2023 | Version Published + Supplemental Material Journal Article Open 1. California Institute of Technology Abstract During a household-transmission field study using COVID-19 antigen rapid diagnostic tests (Ag-RDT), a common test strip lot was identified among three participants with false-positive results. In blinded laboratory evaluation, this lot, exhibited a significantly higher false-positive rate than other lots. Because a positive Ag-RDT result often prompts action, reducing lot-specific false positives can maintain confidence and actionability of true-positive Ag-RDT results. Additional Information © The Author(s) 2023. Published by Oxford University Press on behalf of Infectious Diseases Society of America. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. We thank the study participants for making this work possible. We thank Study Coordinators Saharai Caldera for suggesting the laboratory evaluation and both Saharai and Hannah Davich for removing lot 152000 from circulation in the study. We thank Taikun Yamada, John Raymond B. Reyna, Paolo Piatti and Yap Ching Chew for performing and verifying the validity of RT-qPCR results as previously reported. Finally, we thank all the case investigators and contact tracers at the Pasadena Public Health Department and Caltech Student Wellness Services for their efforts in study recruitment and their work in the pandemic response. This work was supported by the Ronald and Maxine Linde Center for New Initiatives at the California Institute of Technology and the Jacobs Institute for Molecular Engineering for Medicine at the California Institute of Technology. A UCLA DGSOM Geffen Fellowship to AVW. Data Availability: The data underlying the results presented in the study are available at CaltechDATA at: https://doi.org/10.22002/fmz6a-0x036. Alyssa M. Carter and Alexander Viloria Winnett contributed equally to this manuscript. Attached Files Published - ofac701.pdf Supplemental Material - ofac701_supplementary_data.pdf Files ofac701.pdf Files (507.3 kB) Name Size ofac701.pdf md5:2c1716cccecfe2021915a6e0f123c979 337.9 kB Preview Download ofac701_supplementary_data.pdf md5:f3101239a9d910c5dccc5ed24b5c9ac1 169.5 kB Preview Download Additional details PMC9887260 118971 CaltechAUTHORS:20230130-263514000.2 2023-01-30 Created from EPrint's datestamp field 2023-07-05 Created from EPrint's last_modified field COVID-19, Jacobs Institute for Molecular Engineering for Medicine, Division of Biology and Biological Engineering (BBE)
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[Skip to main](https://authors.library.caltech.edu/records/0mjqz-64458#main) [![CaltechAUTHORS](https://authors.library.caltech.edu/static/images/CaltechAUTHORS-by-Caltech-Library-blue-with-padding.svg)](https://authors.library.caltech.edu/) [Communities](https://authors.library.caltech.edu/communities) [My dashboard](https://authors.library.caltech.edu/me/uploads) ![](https://authors.library.caltech.edu/api/communities/aedd135f-227e-4fdf-9476-5b3fd011bac6/logo) [CaltechAUTHORS](https://authors.library.caltech.edu/communities/caltechauthors/records) Published January 30, 2023 \| Version Published + Supplemental Material Journal Article Open # Laboratory Evaluation Links Some False-Positive COVID-19 Antigen Test Results Observed in a Field Study to a Specific Lot of Test Strips ### Creators - [Carter, Alyssa M.1](https://authors.library.caltech.edu/search?q=metadata.creators.person_or_org.name:%22Carter,+Alyssa+M.%22) [![ORCID icon](https://authors.library.caltech.edu/static/images/orcid.svg)](https://orcid.org/0000-0002-2776-9421 "Carter, Alyssa M.'s ORCID profile") - [Winnett, Alexander Viloria1](https://authors.library.caltech.edu/search?q=metadata.creators.person_or_org.name:%22Winnett,+Alexander+Viloria%22) [![ORCID icon](https://authors.library.caltech.edu/static/images/orcid.svg)](https://orcid.org/0000-0002-7338-5605 "Winnett, Alexander Viloria's ORCID profile") - [Romano, Anna E.1](https://authors.library.caltech.edu/search?q=metadata.creators.person_or_org.name:%22Romano,+Anna+E.%22) [![ORCID icon](https://authors.library.caltech.edu/static/images/orcid.svg)](https://orcid.org/0000-0002-7148-0668 "Romano, Anna E.'s ORCID profile") - [Akana, Reid1](https://authors.library.caltech.edu/search?q=metadata.creators.person_or_org.name:%22Akana,+Reid%22) [![ORCID icon](https://authors.library.caltech.edu/static/images/orcid.svg)](https://orcid.org/0000-0003-4422-587X "Akana, Reid's ORCID profile") - [Shelby, Natasha1](https://authors.library.caltech.edu/search?q=metadata.creators.person_or_org.name:%22Shelby,+Natasha%22) [![ORCID icon](https://authors.library.caltech.edu/static/images/orcid.svg)](https://orcid.org/0000-0001-9097-3663 "Shelby, Natasha's ORCID profile") - [Ismagilov, Rustem F.1](https://authors.library.caltech.edu/search?q=metadata.creators.person_or_org.name:%22Ismagilov,+Rustem+F.%22) [![ORCID icon](https://authors.library.caltech.edu/static/images/orcid.svg)](https://orcid.org/0000-0002-3680-4399 "Ismagilov, Rustem F.'s ORCID profile") Show affiliations - 1\. [![ROR icon](https://authors.library.caltech.edu/static/images/ror-icon.svg)](https://ror.org/05dxps055 "California Institute of Technology's ROR profile") California Institute of Technology ## Abstract During a household-transmission field study using COVID-19 antigen rapid diagnostic tests (Ag-RDT), a common test strip lot was identified among three participants with false-positive results. In blinded laboratory evaluation, this lot, exhibited a significantly higher false-positive rate than other lots. Because a positive Ag-RDT result often prompts action, reducing lot-specific false positives can maintain confidence and actionability of true-positive Ag-RDT results. ## Additional Information © The Author(s) 2023. Published by Oxford University Press on behalf of Infectious Diseases Society of America. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. We thank the study participants for making this work possible. We thank Study Coordinators Saharai Caldera for suggesting the laboratory evaluation and both Saharai and Hannah Davich for removing lot 152000 from circulation in the study. We thank Taikun Yamada, John Raymond B. Reyna, Paolo Piatti and Yap Ching Chew for performing and verifying the validity of RT-qPCR results as previously reported. Finally, we thank all the case investigators and contact tracers at the Pasadena Public Health Department and Caltech Student Wellness Services for their efforts in study recruitment and their work in the pandemic response. This work was supported by the Ronald and Maxine Linde Center for New Initiatives at the California Institute of Technology and the Jacobs Institute for Molecular Engineering for Medicine at the California Institute of Technology. A UCLA DGSOM Geffen Fellowship to AVW. Data Availability: The data underlying the results presented in the study are available at CaltechDATA at: https://doi.org/10.22002/fmz6a-0x036. Alyssa M. Carter and Alexander Viloria Winnett contributed equally to this manuscript. ## Attached Files Published - [ofac701.pdf](https://authors.library.caltech.edu/records/0mjqz-64458/files/ofac701.pdf?download=1) Supplemental Material - [ofac701\_supplementary\_data.pdf](https://authors.library.caltech.edu/records/0mjqz-64458/files/ofac701_supplementary_data.pdf?download=1) ## Files ### ofac701.pdf ### Files (507.3 kB) | Name | Size | | |---|---|---| | [ofac701.pdf](https://authors.library.caltech.edu/records/0mjqz-64458/files/ofac701.pdf?download=1) md5:2c1716cccecfe2021915a6e0f123c979 | 337\.9 kB | [Preview](https://authors.library.caltech.edu/records/0mjqz-64458/preview/ofac701.pdf?include_deleted=0) [Download](https://authors.library.caltech.edu/records/0mjqz-64458/files/ofac701.pdf?download=1) | | [ofac701\_supplementary\_data.pdf](https://authors.library.caltech.edu/records/0mjqz-64458/files/ofac701_supplementary_data.pdf?download=1) md5:f3101239a9d910c5dccc5ed24b5c9ac1 | 169\.5 kB | [Preview](https://authors.library.caltech.edu/records/0mjqz-64458/preview/ofac701_supplementary_data.pdf?include_deleted=0) [Download](https://authors.library.caltech.edu/records/0mjqz-64458/files/ofac701_supplementary_data.pdf?download=1) | ## Additional details ### Identifiers PMCID [PMC9887260](https://www.ncbi.nlm.nih.gov/pmc/PMC9887260 "Opens in new tab") Eprint ID 118971 Resolver ID CaltechAUTHORS:20230130-263514000.2 ### Related works Describes [10\.22002/fmz6a-0x036](https://doi.org/10.22002/fmz6a-0x036 "Opens in new tab") (DOI) ### Funding Ronald and Maxine Linde Center for New Initiatives Jacobs Institute for Molecular Engineering for Medicine UCLA ### Dates Created 2023-01-30 Created from EPrint's datestamp field Updated 2023-07-05 Created from EPrint's last\_modified field ### Caltech Custom Metadata Caltech groups COVID-19, Jacobs Institute for Molecular Engineering for Medicine, Division of Biology and Biological Engineering (BBE) 62 Views 44 Downloads Show more details | | All versions | This version | |---|---|---| | Views Total views | 62 | 62 | | Downloads Total downloads | 44 | 44 | | Data volume Total data volume | 13\.4 MB | 13\.4 MB | [More info on how stats are collected....](https://authors.library.caltech.edu/help/statistics) ## Versions ## Keywords and subjects ### Keywords - [Infectious Diseases](https://authors.library.caltech.edu/search?q=metadata.subjects.subject:%22Infectious+Diseases%22 "Search results for Infectious Diseases") - [Oncology](https://authors.library.caltech.edu/search?q=metadata.subjects.subject:%22Oncology%22 "Search results for Oncology") ## Details DOI ![10\.1093/ofid/ofac701](https://authors.library.caltech.edu/badge/DOI/10.1093/ofid/ofac701.svg) DOI Badge #### DOI #### ### Markdown ``` [![DOI](https://authors.library.caltech.edu/badge/DOI/10.1093/ofid/ofac701.svg)](https://doi.org/10.1093/ofid/ofac701) ``` ### reStructuredText ``` .. image:: https://authors.library.caltech.edu/badge/DOI/10.1093/ofid/ofac701.svg :target: https://doi.org/10.1093/ofid/ofac701 ``` ### HTML ``` <a href="https://doi.org/10.1093/ofid/ofac701"><img src="https://authors.library.caltech.edu/badge/DOI/10.1093/ofid/ofac701.svg" alt="DOI"></a> ``` ### Image URL ``` https://authors.library.caltech.edu/badge/DOI/10.1093/ofid/ofac701.svg ``` ### Target URL ``` https://doi.org/10.1093/ofid/ofac701 ``` Resource type Journal Article Publisher Oxford University Press Published in Open Forum Infectious Diseases, 10(1), Art. No. ofac701, ISSN: 2328-8957, 2023. ## Rights #### License - Other No commercial reproduction, distribution, display or performance rights in this work are provided. ## Communities ## Export ## Technical metadata Created August 22, 2023 Modified November 22, 2025 Jump up [About](https://library.caltech.edu/c.php?g=512684&p=11281171) [Policies](https://library.caltech.edu/c.php?g=512684&p=11281170) [Help](https://library.caltech.edu/c.php?g=512684&p=11207017) [Powered by InvenioRDM](https://inveniosoftware.org/products/rdm)
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Published January 30, 2023 \| Version Published + Supplemental Material Journal Article Open - 1\. [![ROR icon](https://authors.library.caltech.edu/static/images/ror-icon.svg)](https://ror.org/05dxps055 "California Institute of Technology's ROR profile") California Institute of Technology ## Abstract During a household-transmission field study using COVID-19 antigen rapid diagnostic tests (Ag-RDT), a common test strip lot was identified among three participants with false-positive results. In blinded laboratory evaluation, this lot, exhibited a significantly higher false-positive rate than other lots. Because a positive Ag-RDT result often prompts action, reducing lot-specific false positives can maintain confidence and actionability of true-positive Ag-RDT results. ## Additional Information © The Author(s) 2023. Published by Oxford University Press on behalf of Infectious Diseases Society of America. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. We thank the study participants for making this work possible. We thank Study Coordinators Saharai Caldera for suggesting the laboratory evaluation and both Saharai and Hannah Davich for removing lot 152000 from circulation in the study. We thank Taikun Yamada, John Raymond B. Reyna, Paolo Piatti and Yap Ching Chew for performing and verifying the validity of RT-qPCR results as previously reported. Finally, we thank all the case investigators and contact tracers at the Pasadena Public Health Department and Caltech Student Wellness Services for their efforts in study recruitment and their work in the pandemic response. This work was supported by the Ronald and Maxine Linde Center for New Initiatives at the California Institute of Technology and the Jacobs Institute for Molecular Engineering for Medicine at the California Institute of Technology. A UCLA DGSOM Geffen Fellowship to AVW. Data Availability: The data underlying the results presented in the study are available at CaltechDATA at: https://doi.org/10.22002/fmz6a-0x036. Alyssa M. Carter and Alexander Viloria Winnett contributed equally to this manuscript. ## Attached Files Published - [ofac701.pdf](https://authors.library.caltech.edu/records/0mjqz-64458/files/ofac701.pdf?download=1) Supplemental Material - [ofac701\_supplementary\_data.pdf](https://authors.library.caltech.edu/records/0mjqz-64458/files/ofac701_supplementary_data.pdf?download=1) ## Files ### ofac701.pdf ### Files (507.3 kB) | Name | Size | | |---|---|---| | [ofac701.pdf](https://authors.library.caltech.edu/records/0mjqz-64458/files/ofac701.pdf?download=1) md5:2c1716cccecfe2021915a6e0f123c979 | 337\.9 kB | [Preview](https://authors.library.caltech.edu/records/0mjqz-64458/preview/ofac701.pdf?include_deleted=0) [Download](https://authors.library.caltech.edu/records/0mjqz-64458/files/ofac701.pdf?download=1) | | [ofac701\_supplementary\_data.pdf](https://authors.library.caltech.edu/records/0mjqz-64458/files/ofac701_supplementary_data.pdf?download=1) md5:f3101239a9d910c5dccc5ed24b5c9ac1 | 169\.5 kB | [Preview](https://authors.library.caltech.edu/records/0mjqz-64458/preview/ofac701_supplementary_data.pdf?include_deleted=0) [Download](https://authors.library.caltech.edu/records/0mjqz-64458/files/ofac701_supplementary_data.pdf?download=1) | ## Additional details [PMC9887260](https://www.ncbi.nlm.nih.gov/pmc/PMC9887260 "Opens in new tab") 118971 CaltechAUTHORS:20230130-263514000.2 2023-01-30 Created from EPrint's datestamp field 2023-07-05 Created from EPrint's last\_modified field COVID-19, Jacobs Institute for Molecular Engineering for Medicine, Division of Biology and Biological Engineering (BBE)
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